Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.3.08 Master Mix

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8.3.08 Master Mix

Reagent Volume/Amount
Colony Suspension 1 μl (not added to master, though)
Taq 0.85 μl
Primer BT2736 6.8 μl 1 μM Stock
Primer BT2737 6.8 μl 1 μM Stock
dNTPs 1.7 μl 10 mM Stock
DMSO 4.25 μl
Buffer 8.5 μl
Mg2+ 4.25 μl
dH20 43.35 μl
Total 76.5 μl

Aliquotted 9 μl of to each of 8 tubes, then added 1 μl appropriate colony suspension.