Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.12.08 Master Mix

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8.12.08 Master Mix

Reagent Volume/Amount
Colony Suspension 9.5 μl (not added to master, though)
Taq 0.95 μl
Primer BT2736 7.6 μl 1 μM Stock
Primer BT2737 7.6 μl 1 μM Stock
dNTPs 1.9 μl 10 mM Stock
DMSO 4.75 μl
Buffer 9.5 μl
Mg2+ 4.75 μl
dH20 48.45 μl
Total 95 μl

Aliquotted 9 μl of to each of 9 tubes, then added 1 μl appropriate colony suspension.