Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.11.08 Master Mix

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8.11.08 Master Mix

Reagent Volume/Amount
Colony Suspension 7.5 μl (not added to master, though)
Taq 0.75 μl
Primer BT2736 6.0 μl 1 μM Stock
Primer BT2737 6.0 μl 1 μM Stock
dNTPs 1.5 μl 10 mM Stock
DMSO 3.75 μl
Buffer 7.5 μl
Mg2+ 3.75 μl
dH20 38.25 μl
Total 75 μl

Aliquotted 9 μl of to each of 7 tubes, then added 1 μl appropriate colony suspension, prep, and genomic DNA template.