Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.11.08 Gel Results
8.11.08 PCR Gel Results
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5||Lane 6||Lane 7||Lane 8|
|5 μl λ ladder||5 μl PCR product pIs001.P7||5 μl PCR product pIs001.P8||5 μl PCR product pIs001.L8 '4'||5 μl PCR product pIs001.L8 '5'||5 μl PCR product pIs001.L8 '9'||5 μl PCR product pIs001.L8 '10'||genomic DNA positive control|
Lanes 2 and 3 (corresponding to P7 and P8 respectively) yielded products at ~4200 bp - Way Off! None of the colony PCRs yielded any product and the positive control worked properly with a band at ~800. Something's gone terribly wrong with the preps.