Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.11.08 Gel Results

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8.11.08 PCR Gel Results

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8
5 μl λ ladder 5 μl PCR product pIs001.P7 5 μl PCR product pIs001.P8 5 μl PCR product pIs001.L8 '4' 5 μl PCR product pIs001.L8 '5' 5 μl PCR product pIs001.L8 '9' 5 μl PCR product pIs001.L8 '10' genomic DNA positive control

Lanes 2 and 3 (corresponding to P7 and P8 respectively) yielded products at ~4200 bp - Way Off! None of the colony PCRs yielded any product and the positive control worked properly with a band at ~800. Something's gone terribly wrong with the preps.