Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/7.25.08 Gel Results
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7.25.08 pIs001.L3 Colony PCR Results
|Lane 1||Lanes 2-8||Lane 9|
|5 μl λ ladder||5 μl pIs001.L3 transformant colony PCRs 1-7||PAO1 Genomic DNA positive control|
120 volts, 20 minutes, 1% Agarose gel. 5 μl of product in each lane. Stained for 15 minutes. Every lane had a band at slightly below the 831 bp band of the ladder. Lane 2 (1st colony) was a bit faint, so I chose 2 and 3 to inoculate overnights.