Matt Gethers/CRI, Thailand/Labwork/Digests/NdeI and BamHI Double Digest/8.5.08 Gel Results

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8.5.08 Digest Gel

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6
5 μl λ maker pET-11a no enzymes pET-11a NdeI pET-11a BamHI pET-11a BamHI/NdeI HmgR ORF BamHI/NdeI

120 V for 30 minutes, 1% gel, stain for 15 minutes in EtBr. Lanes 3, 4, and 5 all differed from lane 2 suggesting that the enzymes are working properly and that the double digest is in fact yielding a doubly cut vector. I excised the ~5 kb band from lane 5 and the ~800 bp band from lane 6. Gel purified, eluted in 50 μl elution buffer.