Kubke Lab:/Notebook/Cranial nerve development/2014/11/19

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Day 3 Attended a lab meeting this morning which was really insightful and gave me a good idea of who was in the lab team and who was working together etc. Unfortunately my dye injections didn’t work even though I tried to clear the nerve stubs as much as possible. I washed the hindbrain In 25% glycerol to make it more transparent but even then I couldn’t see any of the Dio/DiI on the hindbrain under the Leica dissecting microscope. That has spurred me on to realise that I need some assistance from Fabiana Re: whether it’s my dye technique that’s not penetrative enough or my cleaning or whether I’m even injecting nerves so tomorrow we will work together to look under the microscope and I can point out the things I think that I am doing and seeing and she can assist me as to whether they are right or what needs changing.