Kubke Lab:/Notebook/Cranial nerve development/2010/12/20

Cranial Nerve Development

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General Entries Afternoon end of year lab BBQ at Peter Thorne's. Please check your email for details. Personal Entries Malisha On leave --MF Kubke 17:46, 16 December 2010 (EST) Reuben 10:30am Placed slides 1,2,3,4&5 (RC004) which were sectioned on Friday afternoon by me into a staining rack. The rack was then immersed in the staining solutions for the durations provided below: (Note: this entry does not match the embryo page entry --MF Kubke 04:52, 3 February 2011 (EST)) (Note: Do you mean the 70%EtOH time? I will check my notebook on Monday and correct it.) Solution Duration (mins) 70% EtOH 5 95% EtOH 5 100% EtOH 10 Xylene 30 100% EtOH 10 95% EtOH 10 70% EtOH 5 Tap Water 1 Cresyl Violet 15 Tap Water 1 70% EtOH 1 95% EtOH 1 100% EtOH 2 Xylene 20 Coverslipped Infinite (Note: The standard is to do two passes through 100% alcohol and 3 passes through xylene --MF Kubke 05:11, 20 January 2011 (EST)) (Note: Please transfer this information ot the embryo page) Did not complete work in the afternoon due to Christmas arrangements. Fabiana Dealt with lab business in the morning and then went to Peter's BBQ.