Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/26

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26 May 2009 Lab Meeting

† Announcements

  • Please answer my safety emails.
  • PDMS Fixture holder - make more at machine shop.
  • Vacuum chamber needs a new gasket.
  • MicroTAS Abstracts accepted. Papers due June 30th.

† Flu R01

  • RNA extraction troubleshooting.
  • Mass Spec of elution? In Chemistry.- We should approach them.
  • Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA: DID NOT SEE ANYTHING.
  • Absolute Quantification assays: TH this week.
  • FTIR-ATR look at the eluant? There are some organics in the eluant. Restrict the range. Align the laser.

† Coulter Flu Fraunhofer Project

  • Fraun folks coming to get trained next week. RNA isolation.
  • IBC for Fraun flu. in process.

† SEPSIS Project

  • Revision submitted 5/20.

†RNA project

  • Jeff visiting week of June 1st


  • Test with sol-gel substrate w/o bacteria (26th)

  • Evap system (JD and JZ)
    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?

† Virus concentration

† Valve Array

  • Teddy will train Alex. JD. when he back from vacation.

† Fraunhofer: LOAC

  • Paper revised.
  • Bonding ongoing with MIT folks

† Biointerfaces group

  • Final draft with collaborators.
  • Herringbone masks made.
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).

† CIMIT- Colson Grant

  • IRB still in revision.


  • PCR 2 paper draft.
  • will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)


  • Integrated chip worked(19th)
  • 3 more chips are tested when Sonali back(28th Thu).
  • Valve characterization needed

† Silica Optimization (Lambda):

  • Run single PCR product from QQ or Sonali the test with the bioanalyzer