Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/12

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12 May 2009 Lab Meeting

† Announcements

  • New lab meeting time T 10a-12pm room 705 starting 5/12/2009
  • PDMS Fixture holder - make more at machine shop. Follow proper cleaning procedures with PU molds.

† Flu R01

  • RNA extraction troubleshooting.
  • Mass Spec of elution? Martin Steffen?
  • Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA
  • Absolute Quantification assays
  • FTIR-ATR look at the eluant?

† Coulter Flu Fraunhofer Project

  • Fraun folks coming to get trained next week. RNA isolation.
  • IBC for Fraun flu. in process.

† SEPSIS Project

  • Revision in process due 5/20.
  • MM to CMK at the end of this week.

†RNA project

  • Jeff visiting week of June 1st


  • Part last week was leaking b/c of flatness issues. No SERS test.
  • Mask our for SU-8 mold. (PDMS)
  • Test again before next Wed.
  • Evap system (JD and JZ)
    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?

† Virus concentration

  • ATCC flu virus grew and harvested
  • Plaque assay done.

† Valve Array/Valve building - done.

  • SP students are gone? Alex. JD.

† Fraunhofer: LOAC

  • Paper in revision Yeah!
  • Bonding meeting with MIT folks - Alex left some samples with them. Updates?

† Biointerfaces group

  • Final draft with collaborators.
  • designing herringbone groove for better trapping (Mask writer down until next Tuesday)
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).

† CIMIT- Colson Grant

  • IRB still in revision.


  • repeated test of the PCR efficiency for different designs of channel.
  • test PCR efficiency using smaller DNA size (63bp product).
  • analyze PCR product for different temperature using bioanalyzer.
  • getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7)
  • Mincheol has written the introduction, results and discussion, and conclusion.
  • will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)


  • Giving up on white paper. Think about other grant outlets.
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube * Updates?
  • Jane is working on passive valve characterization: threshold pressure not related to cantilever length or bonding time, try hole size next

† Silica Optimization (Lambda):

  • Run single PCR product from QQ or Sonali the test with the bioanalyzer