Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/07

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7 May 2009 Lab Meeting † Announcements New lab meeting time T 10a-12pm room 705 starting 5/12/2009 † Flu R01 Progress report done. sample collection done. . Updates? Three flu fixtures completed today at the machine shop for 400$, another one ordered for general purpose use (Jane and Jaephil) † SEPSIS Project Revision in process due 5/20 †RNA project Jeff visiting week of May 25th † COBRA Integration with the glass slide. new air channel design sent to machine shop new fluid channel made in PDMS making the polyurethane mold had proven challenging as it sticks to the plastic fixture, tried mold release but didn't help new cutting plotter carrier sheet ordered new air channel mold did not cure well and the PDMS made from it was leaking. need to do it again, mold material ordered. Substrates (JZ) Let's get this to a point where we can publish and move on....JZ and CMK to discuss. - Making substrate in microwewlls? (motivation? need to implement valves) - Co-evaporate colloids? (the morphology won't be uniform or consistent each time, signal analysis system non-existent) - Sol-gel on Teflon? (can serve to study basic material science only) Colloid experiment - Do Rhodamine. Leaked. Need to try again. - Found the problem. Liquid and air channel widths reversed. Will make new chip and try again. -Need a size specific positive control. PS beads? charged? 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate try coated PS beads. † Virus concentration ATCC flu virus grew and harvested Plaque assay of harvested virus in progress: learning from Brendan, will try if enough cells Concentrator chip design underway † Valve Array/Valve building - done. Who is in charge of this now that the SP students are gone? Alex? † Fraunhofer: LOAC Paper in revision Yeah! Bonding meeting with MIT folks - Alex left some samples with them. Updates? † Biointerfaces group Final draft with collaborators. designing herringbone groove for better trapping (Mask writer down until next Tuesday) investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force). † CIMIT- Colson Grant IRB still in revision. † PCR Cathie read and gave back PCR 1 paper to QQ and MCK Qingqing has written the introduction repeated test of the PCR efficiency for different designs of channel. test PCR efficiency using smaller DNA size (63bp product). analyze PCR product for different temperature using bioanalyzer. getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7) Mincheol has written the introduction, results and discussion, and conclusion. will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) † RCA/HDA Giving up on white paper. Think about other grant outlets. Preliminary no-valve design for 25 microliters. 20 more test chips to Sonali (AACC poster). Sonali try to do 10 microliters in the tube * Updates? Jane is working on passive valve characterization: threshold pressure not related to cantilever length or bonding time, try hole size next † Senior project Megan HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run. Plasma helped bonding. EHS wants MSDS stuff. † Silica Optimization (Lambda): Run single PCR product from QQ or Sonali the test with the bioanalyzer