Khosla:pCK-KPY293

pCK-KPY293

TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCAGATCTCAATTGGATA
TCGGCCGGCCGCAGTGGTGACAATGGGATGACTGAGGAAAAACTGCGTCGCTACCTGAAACGTACTGTGACAGAATTGGATAGTGTGACAGCTCGCCTTCGTGAGGTTG
AGCACCGTGCAGGAGACCCAATTGTGATTGTTGGAATGTCATGCCGCTTCCCTGGCGGCGTCCGCACACCCGAGGATTTCTGGGACTTACTTTGGCACGGGCGTGACGC
TATCACGGAGGTACCACCCCAGCGCTGGGATATCGACGACTATTACGACCCAGACCCAGCTGCGTTGGGTACCATGTACACTCGCTGGGGCGGTTTTGTGGATGGCGCT
GATGAATTTGATCCAGCCTTTTTCGGCATTGGGGCTGCGGAAGCTCGCGCCATGGACCCGCAGCAACGCCTTCTGCTTGAGACATCCTGGGAAGCATTAGAGCGCTCAG
GACGCGCGCCAGGTGCACTGGCGGGGTCTCGCACTGGCGTTTTTGTGGGTCTTTGTTTTTCCGAATATCCCGCAACGCCCATCCGTGCCGACGATCCAACGGCAATCGG
CGCCTACTCGGTCATTGGGTCGGCACCGTCTGTCGCGGCCGGTCGCCTGGCTTACGTGTTAGGCCTTCAAGGGCCTGCCATGACCCTTGACACTGCATGCAGCTCCAGC
TTAGTAGCATTACAAGCCGCAGCAGATGGATTGCGTGCCGGTCGTTGTGATTTAGCGTTGGTAGGAGGCGTAAACCTGCAACTGGCTCCCGAGACCACGATTGGATTTT
GTCGTTTGGCATCGCTGTCACCCGACGGACGTAGTCGCGCTTTTGACGCGGATGCCAATGGGTACGTACGTGCCGATGGATGCGGAGTTTTGGTGTTGACTCGTTTGTC
TCAGGCGCGTCGCAACGGCGACCGCGTTCTTGCGATCGTGCGCGGCGTAGCAGTGAACCACGATGGCCGCTCAAACGGCTTAACCGCTCCCAATGGCCCAGCGCAACAG
CGTGTAATCACTGATGCCCTGGAACGTGCTGGACTGCGCCCGGATGCAGTCGACTACGTGGAAACCCATGGCACAGGGACGCCTTTAGGGGATCCCATCGAGGCCACTG
CCTTGGCAGAGGTGTACGGGGCCAACCGCGCACAACCCCTGTTAATCGGTAGTGTAAAAACAAATATCGGCCACACTGAAGCAGCAGCTGGAGTCGCAGGTGTGATCAA
AGCGGTGTTAATGCTTCGTCACCGCCGTATCCCGCCATCATTGCATTTCCATACACCAAACCCACTTATTGACTGGGCAGGATTGCCCTTAGAGGTGGTCCGTGAAGCC
CGCGATTGGCCGCGTACAGAACATGCTCCGGCGATTGGAGTCAGTTCATTTGGGATGTCGGGGACAAACGCGCACGTGATTCTGGCGCAGGGGGACCCTGCACCTGCTC
CACGTCCGGAGTCACCGGCTGCGCAAGTCCTGGTGTTGAGCGCACGTGACGAGACTGCACTTGCGGATATCGCCCGCCGCTGGGCGGATGAGATCGAAAGTGCACGTGC
AGCTTTCGGAGACCTGGCATATACCGCGGCAACAGCACGCACCCACTTTGAGGAACGTCTGGCAGTTGTGGCGGATTCCGGTGCTGCGGCGGCGCGCGACCTGCGCGCT
CCCATCCCCATCCGCGGTCGCGTCGAACGCACAGCGCGCGCTAAAGTTGCGTTGCTGTTTACTGGACAAGGCAGCCAGTACCCGGGTATGGCTCGCGAATTGTTTGATT
CAGAACCGGTGTTTCGTGATGCTCTGCGCCGTTGCGACGCAGTTCTGGGCCCAATTGCCGACGGAGTTGGATTAATCGATGCCGTTTACGGCGACCACGGTCCGGAGTC
ACTTCGCCGCACTGTATTTACACAACCAGTCCTTTTTGCTGTTGAATGGGCATTATGGGAGTTGTGGCGCGCCTGGGGAATTGCCCCTGCCGCCGTCTTGGGACATTCG
GTGGGGGAGTATGTCGCGGCATGCGCCGCGGGAGCCATGACCGTTGAGGATGCGCTTCGCCTTGTAGCTGTTCGTGGTCGCCTGATGCAGCGTTTACCGGATGGTGGAG
GTATGATGGCCGTGGATGCTTCAGCGGAACAAGTGGCGCCTCTGGTTGCACGCCATGAACCCGCCTTGAGCATCGCAGGGTATAACGGTCCTGCACAAGTCGTCGTAGC
TGGTCCGCGTGCGGCATTAGACCTTCTGCGCGAACAGTTAAGTGCTGACGGCATCCGTTCCGCGGACTTAACCGTCAGCCACGCATTTCATAGTGCAGCCATGGATCCG
ATCCTGGATGATCTTGCCGTCGCAGCATCGCGCGCAACGATGACAGACCCAGCTATCCCCTTGATCTCAAATTTGACCGGGGCTCCTTTACGTCCAGGAGAGCTGGGGC
CACGTTATTGGGCTCGTCACGCCCGTCAGCCTGTGCGCTTCGCGCAGGGGTTACGCGCGATGGCAGAGCTTGGTATCGATCAGTTTTTAGAAATCGGGCCGCATCCCAC
ACTGTCCGCACTGGGGGGACGCGTCTTGGGAGATTCGGCCCGTTTTATTGCGAGCCTTCGCCGCGGTCGCGGGGATCGCGAAACCATGATGACGGCATTAGGTAATTTA
TACGTAGGGGGCGTGGCGGTAGATTGGGAACAAGTCCACCGCCCACACGATCGTCGTCGTGTACCCGGGCCCACCTATCCCTTTCAGCGTCAACGCTATTGGGTCGAAT
CGCACCTTGCGGCCGTACCGCCGAGCGTAGATGTAGACGCCGTGTCTGAGGCGAGTCTTCGCGTACGTCGTGGTATCGCTGCTGCGGCTGACGAACAAGAGGAGGACCC
AAGTTCACCCGAGGGAACAACGTATTTTAACCTTACTCTGGGTCCCGATAGTCCCTCATACCTGGCAGACCATGTGGTGGATCGCACCGTGGTGGTCCCAGGCGCGTGG
TTCGTGGCCACAATCGCAGATATTGCTCGCGGGTTGTTGGCAGATGACGTGGTAGCAATCGAGGACCTTGTCATCACATCGGGGTTAGCGCTGACCGGCCCTCAACCTG
TTCGTATTGTCTTTGAACCGACTGGCGATGGGTTTGCGGTGCACGTCCAGACGGAGTCCTCGGGGACCCACGCCCGTGCGCAAGTTCGTACTGGTACCCACCGCTTGCC
AGATGGAGCTGGATTAGCAGAGATTCTTGACCATTGTCCTGAGCACTTAGCTCCGGAGGATTTTTATCAACGTTTAGCGCGCGCAGGATTACCATACGGGCCCGCCTTC
CGCCGCGTAGTTGGTTTGCGCCTTGGCCGCCATGAAGCTCTTGCCACGTTGTCGGATTTCGATACAGATACCGGTACAGTCCACCCGGCGCTTTTAGACGCGGCCTTCC
AGGCCACCTACGCTGCAATGTCGCCGCGCGCTGACGAGCAGACCTGGGTCCCTTTCGCGGTAGACCAAGTCCTGGCATCTGCTGCCGTCGGAACTGTTCGTTTTGGTCA
TGTTCGTGTTCTGGAACAGACCCCGCAATTGTGTGTAGCGGACGTGCGTTTATTGGATGAAGAAGGATCGCCCGTACTTGTCGCAAACGGCCTGCGTGTAATGCCGGCC
CCTACACCCAATTCTGCAGGCCCCTCTTCCCATGAGCGCTCCGTGGCGCACACATACCGTATCGATTGGCGCGAGACGGGGAGTGCAGCGCCGGCAAGTGCAGCCGGTC
GTTGGTTGATCATCGGTGCGCCGGGGTCCTTTGCCGATTCCCTGGCTGAGGATCTTCGTGCACGTGGCGGTGAGTGTGTTATCGCTCGCCCGGGAACTGGATTCACCTT
TCGTGCGGCCAACGAATTTGAGATTGATTTAGGAGTGCCGGCTGCGGTACGCGCTCTTTTGGGTGCGGTAGATGATGGTCGTGCTCCATTGCGTGGTATCGTCTCCGCG
GTTGCATTGAGTGAACGCAACGCGCCAGCACCAGTTGCTGCTGAACGTTTGGTCGTGGGGGCCTTACACCTTGTCCAAGCAGCTGCTTTAGCGGGTGAACCCGCCGTAT
GGCTGCTGACCCGTCACGGTCAACGTGTGGAGCCAGCAGACCGTGTTGCACCGCAACAGTCTGCTCTTTGGGGATTCGCGCGCAGTGCCCAGCTTGAACATCGTGCAAT
TGGGTGCCATGTAATTGATATCGATCAAGTGACCGACGTCCGTTCCGTGGTGGATGCCCTGGTAACGCCGTCTGCTCCGCGCCAACTTGCCTTACGTCAGGGCCGTCGT
CGCGTGCCACGTTTAGTTCCATTAACAGTCGTACCTGGTGAGCCGACGGCCACGGGAGGTACAGTCCTGATTACAGGGGGCTTGGGGGGCGTCGGACTGCGTTTGGCGC
GCTGGTTCGTTGACCGTGGGCGCCCGGACATTGTGCTGGCTGGACGTTCAGCCCCGGACGCGCGCGCACAGGCGGTGATTGCTGAACTGCGCGGACGCGGCGCTCGTAT
CGAAGCACGCTCCCTTGATGTGGCTGATCGTGCTGCAGTAGCCGCTACACTTGACTGGATTGATCGCGAGTTACCCCCGCTTGCCGGAGTAGCTCATCTTGCGGGTGTC
CTTGACGATGGTGTTATCGCGGAGCAGACCGCGGCTCGTGTCGGTCGCGTATTGGCGCCCAAAACTTTCGGAGCGTGGCATCTGCACGAGTTGACAGCTGGCCGTGCTT
TGGAGTTCTTCCTGCTGTTCTCCTCCGCGGCTGCTGTTGTCGGTTTACCCGGTCAATCGGGGTACGCGGCAGCCAATGCTTTCTTAGATGGGATGGCAGAATCTCGTGG
GGCCTCTGGGTCTCCGGCACTGTCCATTGCATGGGGTCCTTGGGCAGACACAGGCATGGTCGAGCGCCTGGATTCAGCCGCCGTGCGCCGCCTGAATCGCCGTGGATAC
GGATTACTTCGCCCGGAGCACGCGTTCGATACCTTGGATCGTCTTATTGGGTCAGGGCCCGCCACTGTTACTACCATGCCACTGACTCTTAGCGGAGTTTCTGACGTTG
ATGTCCCTGATGTCCTGCTGGATAGCGTAGCAACGACGGTCGAAAAGGACAGTTCGACAGCGGCGTCCGCGGATTTGAGTGCGCGCATGGAAGCACTTGTCCGTCGCTG
CTTGGGTTTAGCACCGGGAGATGATCTGGACCGTCCCCTGCACGAGTTAGGGTTAGATTCCGTTGCGGCTTTAGAAATTCGTGATGAGTTATCTCGTTCTTCCGGCCAT
CGTTTACCCGCCACGTTGGCTTTCGAGTATCCCACTCCACGCGCGGTGGTCGGTTTGTTGGTTCGTTTAGGGGGGACAGAGGTCCGCGGCGAAGCGCCCTCGGCATTGG
CCGGGTTAGACGCGCTGGAGGCGGCGCTTCCAGAGGTTCCAGCGACGGAACGCGAGGAGCTGGTACAGCGCCTGGAACGTATGCTGGCTGCTTTACGTCCTGTAGCACA
AGCGGCAGATGCGTCGGGGACCGGCGCTAACCCATCCGGAGACGATTTAGGAGAGGCCGGTGTAGACGAGTTATTAGAAGCCCTGGGTCGTGAGTTGGACGGTGATGGA
TCGTGGAGCCACCCGCAGTTCGAAAAATAACTAGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTG
AAACCTCAGGCATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGA
CGACAAGCTGACGACCGGGTCTCCGCAAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA
ATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAA
ACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTT
ATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCA
AAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGGTCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACC
GGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGC
ATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTG
CCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAG
CATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCT
CATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGCATGCTAGCGCAGAAACGTCCTAGAAGATGCCAGGAGGATACTTAGCAGAGAGACAATAAG
GCCGGAGCGAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGACGAACATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGATGGCTCCCTCTTGCGCTCTCCTGTTCCCGTCCTGCGGCGTCCGTGTTGTGGTGGAGGCTTTACCCAAATCACCACGTCCCGTTCCGTGTAGACAGT
TCGCTCCAAGCTGGGCTGTGTGCAAGAACCCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTATCATCTTGAGTCCAACCCGGAAAGACACGACAAAACGCCA
CTGGCAGCAGCCATTGGTAACTGAGAATTAGTGGATTTAGATATCGAGAGTCTTGAAGTGGTGGCCTAACAGAGGCTACACTGAAAGGACAGTATTTGGTATCTGCGCT
CCACTAAAGCCAGTTACCAGGTTAAGCAGTTCCCCAACTGACTTAACCTTCGATCAAACCGCCTCCCCAGGCGGTTTTTTCGTTTACAGAGCAGGAGATTACGACGATC
GTAAAAGGATCTCAAGAAGATCCTTTACGGATTCCCGACACCATCACTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATAT
AAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTA
ACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT
GGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGG
ACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGA
CATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGG
CCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAG
AGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAG
AAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATT
TGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCA
TCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAA
CGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTC
TCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC
CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAA
CCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAAT;

https://benchling.com/s/seq-oOVsdJ5vT3PaF9I7Vi70