JLogan; saga MOD 3

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Ada subunits size,chromosome,null p-type notes
Ada1 (aka HFI1, SUP110, SRM12, GAN1) 1.467 kb/489 aa, Chr. XVI,
viable
==gene product==

==molecular function==

systematic deletion

Ada2 (aka SWI8) 1.305 kb/434aa, Chr. IV,
viable
==gene product==: Ada histone acetyltransferase complex component,

transcriptional coactivator
==molecular function==: transcription coactivator activity (TAS)
==systematic deletion==:

  • viable
  • decreased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
Ada3(aka NGG1, SWI7) 2.109 kb/702aa, Chr. IV,
viable
==gene product==

==molecular function==

systematic deletion

Gcn5 (aka ADA4, SWI9) 1.32 kb/439aa, Chr. VII,
viable
==gene product== Ada histone acetyltransferase complex component

molecular function

  • histone acetyltransferase activity (IDA)
  • transcription coactivator activity (TAS)

systematic deletion

  • reduced fitness in rich medium (YPD)
Ada5 (aka SPT20) 1.815 kb/604aa, Chr. XV,
viable
same as SPT20
Spt subunits size, chromosome, null p-type notes
Spt3 1.014 kb/337aa, Chr. IV,
viable
==gene product==

==molecular function==

systematic deletion

Spt7(aka GIT2) 3.999 kb/1332aa, Chr. II,
viable
==gene product== histone acetyltransferase SAGA complex member

transcription factor
==molecular function== * structural molecule activity (IDA, IMP)

systematic deletion

  • viable
  • increased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
Spt8 1.809 kb/602aa, Chr. XII,
viable
==gene product== transcription factor

molecular function

  • TATA-binding protein binding (IDA, IMP)
  • transcription cofactor activity (IMP, IPI)

Systematic deletion

  • viable
  • Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium.
  • normal chitin levels
  • increased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
Spt20 (aka Ada5) 1.815 kb/604aa, Chr. XV,
viable
==gene product== histone acetyltransferase SAGA complex member

transcription factor
==molecular function== transcription cofactor activity (IGI, IMP, IPI)

systematic deletions

  • decreased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
TAF subunits size, chromosome, null p-type notes
TAF5 (aka TAF90) 2.397 kb/798aa, Chr. II, inviable ==gene product==

==molecular function==

systematic deletion

TAF6 (aka TAF60) 1.551 kb/516aa, Chr. VII, inviable ==gene product== TATA-binding protein-associated-factor

TafII60
==molecular function== * general RNA polymerase II transcription factor activity (TAS)
==systematic deletion== * inviable

TAF9 (aka TAF17) 0.474 kb/157aa, Chr. XIII, inviable ==gene product== TFIID subunit

TafII17
==molecular function== * general RNA polymerase II transcription factor activity (TAS)
==systematic deletion== * inviable

TAF10 (aka TAF23, TAF25) 0.621 kb/206aa, Chr. IV, inviable ==gene product== TFIID subunit

TafII25
==molecular function== * general RNA polymerase II transcription factor activity (TAS)
==systematic deletion== * inviable

TAF12(aka TAF61, TAF68) 1.620 kb/539aa, Chr. IV, inviable ==gene product== TFIID subunit

TafII61 TafII68
==molecular function== * general RNA polymerase II transcription factor activity (TAS)
==systematic deletion== inviable

Tra1 subunit size, chromosome, null p-type notes
Tra1 11.235 kb/3744aa, Chr. VIII, inviable Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation (1, 2, 3, 4)

systematic deletion

other subunits size, chromosome, null p-type notes
Sgf73 1.974 kb/657aa, Chr. VII ,
viable
3 kDa subunit of SAGA histone acetyltransferase complex; involved in formation of the preinitiation complex assembly at promoters (1, 3)

==systematic deletion== viable

Sgf29 0.779 kb/259aa, Chr. III,
viable
SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex (1)

==systematic deletion== viable

  • Exhibits sensitivity at 15 generations when grown in medium of pH 8.
Sgf11 0.3 kb/99aa, Chr.XVI,
viable
Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation (1, 2)

==systematic deletion== viable

Ubp8 1.416 kb/471aa, Chr. XIII,
viable
Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B (1, 2)

==systematic deletion== viable

Sus1 gene with intron, Chr. II,
viable
Protein involved in mRNA export coupled transcription activation; component of the SAGA histone acetylase complex (1)

systematic deletion