JCA pBca1256-K112012.seq

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LOCUS pBca1256 2982 bp ds-DNA linear 31-JAN-2009 DEFINITION . ACCESSION VERSION SOURCE .

 ORGANISM  .

COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers

    primer_bind     44..61
                    /label=M13-fwd
                    /ApEinfo_fwdcolor=cyan
                    /ApEinfo_revcolor=green
    primer_bind     complement(997..1017)
                    /label=M13-rev
                    /ApEinfo_fwdcolor=cyan
                    /ApEinfo_revcolor=green
    misc_recomb     77..176
                    /label=AttL1
                    /ApEinfo_fwdcolor=plum
                    /ApEinfo_revcolor=plum
    misc_feature    756..761
                    /label=BamHI
                    /ApEinfo_fwdcolor=#00ff00
                    /ApEinfo_revcolor=#00ff00
    misc_feature    1089..1094
                    /label=XbaI
                    /ApEinfo_fwdcolor=#00ffff
                    /ApEinfo_revcolor=#00ffff
    misc_feature    246..251
                    /label=EcoRI
                    /ApEinfo_fwdcolor=#0080ff
                    /ApEinfo_revcolor=#0080ff
    misc_feature    255..260
                    /label=BglII
                    /ApEinfo_fwdcolor=#00ff80
                    /ApEinfo_revcolor=#00ff80
    misc_feature    2242..2247
                    /label=XhoI
                    /ApEinfo_fwdcolor=#00ff00
                    /ApEinfo_revcolor=#00ff00
    misc_feature    complement(834..853)
                    /label=G00101
                    /ApEinfo_fwdcolor=#99e4fb
                    /ApEinfo_revcolor=#99e4fb
    misc_feature    185..206
                    /label=CA998
                    /ApEinfo_fwdcolor=#9bd1f9
                    /ApEinfo_revcolor=#9bd1f9
    misc_feature    861..960
                    /label=attL2
                    /ApEinfo_fwdcolor=#e9b4fa
                    /ApEinfo_revcolor=#e9b4fa
    misc_feature    1229..2239
                    /label=specR
                    /ApEinfo_fwdcolor=#f2f09d
                    /ApEinfo_revcolor=#f2f09d
    misc_feature    2253..2919
                    /label=colE1
                    /ApEinfo_fwdcolor=#c0c0c0
                    /ApEinfo_revcolor=#c0c0c0
    misc_feature    261..755
                    /label=T4 Lysozyme
                    /ApEinfo_fwdcolor=#ff0080
                    /ApEinfo_revcolor=#ff0080

ORIGIN

       1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTcct aggTGTAAAA CGACGGCCAG
      61 TCTTAAGCTC GGGCCCCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA
     121 ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAACTTTG TACAAAAAAG CAGGCTCCGA
     181 ATTGgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     241 ttctgGAATT CATGAGATCT atgaatatat ttgaaatgtt acgtatagat gaaggtctta
     301 gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt catttgctta
     361 caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt gggcgtaatt
     421 gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat gttgatgctg
     481 ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct cttgatgcgg
     541 ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt gtggcaggat
     601 ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca gttaacttag
     661 ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt acaacgttta
     721 gaactggcac ttgggacgcg tataaaaatc tataaGGATC CtaaCTCGAc gtgcaggctt
     781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     841 caaaggcggt aatCAATTCG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAAAAATAA
     901 TTGCTCATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG
     961 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA
    1021 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC
    1081 ATGCCTCCTC TAGACCAGCC AGGACAGAAA TGCCTCGACT TCGCTGCTGC CCAAGGTTGC
    1141 CGGGTGACGC ACACCGTGGA AACGGATGAA GGCACGAACC CAGTGGACAT AAGCCTGTTC
    1201 GGTTCGTAAG CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA GAACCTTGAC
    1261 CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG ACTGTTTTTT
    1321 TGGGGTACAG TCTATGCCTC GGGCATCCAA GCAGCAAGCG CGTTACGCCG TGGGTCGATG
    1381 TTTGATGTTA TGGAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT
    1441 AAACATCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT CAACTATCAG AGGTAGTTGG
    1501 CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA CATTTGTACG GCTCCGCAGT
    1561 GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG GTTACGGTGA CCGTAAGGCT
    1621 TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG GAAACTTCGG CTTCCCCTGG
    1681 AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT GTGCACGACG ACATCATTCC
    1741 GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA TGGCAGCGCA ATGACATTCT
    1801 TGCAGGTATC TTCGAGCCAG CCACGATCGA CATTGATCTG GCTATCTTGC TGACAAAAGC
    1861 AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG GAACTCTTTG ATCCGGTTCC
    1921 TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG CTATGGAACT CGCCGCCCGA
    1981 CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC CGCATTTGGT ACAGCGCAGT
    2041 AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG GCAATGGAGC GCCTGCCGGC
    2101 CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT CTTGGACAAG AAGAAGATCG
    2161 CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC TACGTGAAAG GCGAGATCAC
    2221 CAAGGTAGTC GGCAAATAAC CCTCGAGCCA CCCATGACCA AAATCCCTTA ACGTGAGTTA
    2281 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
    2341 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
    2401 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
    2461 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
    2521 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
    2581 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
    2641 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
    2701 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
    2761 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
    2821 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
    2881 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
    2941 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT 

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