IGEM:metu/2009/Notebook/wound dressing/2009/10/18

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18.10.2009

(Group 2)

1.Directly testing the oxygen promoter in the presence or absence of oxygen.We have prepared 4 set of tubes in the presence or absence of oxygen and we compared the growth of bacterias by using spectrophotometer. OR, testing the hemoglobin activity; we thought that if the gene coding for hemoglobin is working it means that our oxygen promoter is also working.

(Group 1)

2. Centrifuge of supernatant + ammonium sulfate, 14000 rpm for 30 minutes.

3. Preparation of potassium phosphate buffer ( pH 6.6)

4. Resuspend the pellet in potassium phosphate buffer, 5ml,0.1 M and pH 6.6

5.Dialyzing them (Lard, Lard + ABC and Only top 10) in a nitrocellulose tube at 4 centigrade degrees in 500 microliter potassium phosphate buffer stirring for overnight.