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1. We did not have enough TCA so we decided to make ammonium sulfate Precipitation. We found the substances which are necessary for this procedure.
2. 11:30 --- Centrifuge of LB+ top 10 and LB+ Lard1.
we took the supernatant and put the tubes -20 °C.
17:00 --- Again we centrifugated our substances at 12000 rpm for 15 min.
17:20 --- We add 60 % ammonium sulfate to supernatant that we obtained yesterday. Inbubation at 4 °C for overnight.