IGEM:metu/2009/Notebook/wound dressing/2009/10/10

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10.10.2009

1. Monodigest of EGF,pO,KGF,Lard 1, Granulysin,Backbone 4 and Backbone 9 with EcorI. We used Buffer O. After waiting 4 hours, we made inactivation of enzyme by incubation at 65 C for 20 minutes.

2. Purification of digested EGF,pO,KGF,Lard 1, Granulysin,Backbone 4 and Backbone 9.