IGEM:metu/2009/Notebook/wound dressing/2009/10/07

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07.10.2009

1. We prepared LB Broth. (190 ml,250 ml, 250 ml and 250 ml) They were named as Lard1+ABC (1), Lard1+ABC (2), Lard1 (1) and Lard1 (2).

In Lard1+ABC (1) ; 190 μl Ampicillin , 646 μl chloramphenicol and 200 μl bacteria with Lard1+ ABC were add.
In Lard1+ABC (2) ; 250 μl Ampicillin , 850 μl chloramphenicol and 200 μl bacteria with Lard1+ ABC were add.
In Lard1 (1) ; 250 μl Ampicillin and 200 μl bacteria with Lard1 were add.
In Lard1 (2) ; 250 μl Ampicillin and 200 μl bacteria with Lard1 were add.

We put these flasks into shaker , 37 C, 190 rpm.