IGEM:metu/2009/Notebook/wound dressing/2009/09/15

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15.09.2009

1. Double digest(fast digest) of granulysin, KGF, Lard 1 and Backbone

Plasmid 4th was monodigested with XbaI.

Double digest of Poxygen with XbaI and SpeI

For KGF;
5 microliter DNA
2 microliter SpeI
0.5 microliter EcorI
2.5 microliter Buffer R
15 microliter water nuclease free
For Granulysin;
15 microliters DNA
3  microliter Buffer O
1.5 microliter PstI
1.5 microliter EcorI
9 microliter water
total: 30 microliter
For Lard1;
15 microliters DNA
3  microliter Buffer O
1.5 microliter PstI
1.5 microliter EcorI
9 microliter water
For BB;
10 microliter DNA
1 microliter EcorI
1 microliter PstI
2 microliter Buffer O
6 microliter water nuclease free