|WOUND DRESSING||Main project page|
Previous entry Next entry
1. Electrophoresis was done. The plasmids which are numerized as 1,2,6,8, BBa, BBb and BBc were load into gel. Plasmids were about 28 microliter so 5 microliter loading dey was put.
2.Double digestion of EGF,KGF,Lard1, Granulysin and Poxygen were done.
For EGF,KGF, Granulysin and Poxygen; water - 24 microliter Buffer H - 4 microliter DNA - 10 microliter Enzymes - 1 microliter EcoRI and 1 microliter SpeI
For Lard1; water - 19 microliter Buffer H - 4 microliter DNA - 15 microliter Enzymes - 1 microliter XbaI and 1 microliter pstI