IGEM:metu/2009/Notebook/wound dressing/2009/09/12

From OpenWetWare
Jump to navigationJump to search
WOUND DRESSING Main project page
Previous entry      Next entry

12.09.2009

1. Electrophoresis was done. The plasmids which are numerized as 1,2,6,8, BBa, BBb and BBc were load into gel. Plasmids were about 28 microliter so 5 microliter loading dey was put.

2.Double digestion of EGF,KGF,Lard1, Granulysin and Poxygen were done. 

For EGF,KGF, Granulysin and Poxygen;
water    - 24 microliter
Buffer H - 4  microliter
DNA      - 10 microliter
Enzymes  - 1 microliter EcoRI and 1 microliter SpeI

For Lard1;
water    - 19 microliter
Buffer H - 4  microliter
DNA      - 15 microliter
Enzymes  - 1 microliter XbaI and 1 microliter pstI
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:metu/2009/Notebook/wound_dressing/2009/09/12&oldid=1002707"