IGEM:metu/2009/Notebook/wound dressing/2009/08/22

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22.08.2009

1. Isolations of LUXR-RBS(2), Lysis, AHL, BB were made.

2. Digestions of BB,LUXR-RBS AND KGF4 were made.

3. We couldn't digest.

4. Transformations of 1,2,3,4,5,6,7,8,9,10,11 were made. We saw white colonies.

5. Plasmid order:

 1- rbs-tetR-dbt    BBa-P0440
    2007-plate1-21K
    2009-plate1-10I    840bp
    2079bp plasmid
 2- ptetR-RBS     BBa-J13002
     2007-plate2-3F
     2009-plate1-BB   74bp
     2079bp plasmid
 3- rbs-GFP-dbt    BBa-I13504 (EGF4 (GFP)
      2009-plate1-22I    875bp
      2079 bp plasmid
 4- rbs-GFP-dbt  BBa-E0240
      2009-plate1-12M    876bp
      2007 plate 1-16A
 5- lae1+pl-rbs-luxr-dbTT-pluXR    BBa-F2622 
      2007-plate1-6O
      2009-plate1-12G   1062bp
      2079bp plasmid
 6- Pqrr4     BBa-K131017
      2009-plate3-8E   275bp
      2079bp plasmid
 7- pcılamBda-rbs     BBa-K081006 
      2009-plate2-10H    72bp
 8- rbs-cılamBda-dbTT    930bp   BBa-P0451
      PaPs (Cl lambda)  (S0153)
      2009-plate2-1N
      2007-plate1-210
 9- rbs-mRFPI-dpt   861bp   RFP-BBa-I13507
      2007-plate1-16N
      2009-plate 1-220
 10- lysis 20 M
 11- lysis 20K