IGEM:metu/2009/Notebook/wound dressing/2009/07/16

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  • Transformation was successful:)
LB Agar-Amp-Xgal-IPTG- Freeze-Dry =  blue colonies
LB Agar-Amp-Xgal-IPTG- Backbone =  red colonies
LB Agar-Amp-Freeze Dry = white colonies
LB Agar- Amp-Xgal- IPTG – control plasmid = blue colonies
  • For storage and plasmid isolation, single colonies were taken from each plate and put them to incubation at 320 rpm, 16 hours.