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Compotent cell culture was taken from incubation. Process of growing competent cell continued.
result = 0.24
Our results ; 1.flask = 0.67 2.flask = 0.71 3.flask = 0.74 we used first flask and kept on process.
for 10 mM = 0.044g CaCl2 + 40 ml dH2O for 75 mM = 0.8325g CaCl2 + 10 ml dH2O
10 ml dH2O + 1 gr. Amp