IGEM:metu/2009/Notebook/wound dressing/2009/07/08

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08.07.2009

Compotent cell culture was taken from incubation. Process of growing competent cell continued.

  • 50 ml. LB was put in 3 flasks.
  • 300µl bacteria solution which is waited in incubation for 14- 16 hours is put in each flask.
  • Again incubation at 320 rpm for 2-2.5 hours.
  • After 2 hours we measured OD (600 nm) with spectrophotometer.
result = 0.24  
  • So we continued incubation because we want result of OD is about 0.6
  • After 1.5 hours, we measured again.
Our results ; 
1.flask = 0.67 
2.flask = 0.71
3.flask = 0.74
we used first flask and kept on process.
  • 10 mM and 75 mM CaCl2 was prepared.
for 10 mM = 0.044g CaCl2 + 40 ml dH2O 
for 75 mM = 0.8325g CaCl2 + 10 ml dH2O 
  • ampicilline was made.
10 ml dH2O + 1 gr. Amp
  • LB Agar was prepared.