IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/23

iGEM Project name 1

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July 23rd, 2010 Held SKYPE conference with subteams Measured fluorescence of CFP and TOP10 cells with new CFP filter Characterized spectrum of EGFP with spectrofluorometer Read fluorescence of cotransformation (RFP and GFP) cells with spectrofluorometer Minipreped CFP constructs for gel electrophoresis Quantified DNA of CFP minipreps Digested CFP construct plasmids Ran gel electrophoresis on CFP construct plasmid DNA Excised two bands of gel DNA for purification Purified DNA from gel bands Autoclaved glassware and supplies Cleaned lab