IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/23

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July 23rd, 2010

  • Held SKYPE conference with subteams
  • Measured fluorescence of CFP and TOP10 cells with new CFP filter
  • Characterized spectrum of EGFP with spectrofluorometer
  • Read fluorescence of cotransformation (RFP and GFP) cells with spectrofluorometer
  • Minipreped CFP constructs for gel electrophoresis
  • Quantified DNA of CFP minipreps
  • Digested CFP construct plasmids
  • Ran gel electrophoresis on CFP construct plasmid DNA
  • Excised two bands of gel DNA for purification
  • Purified DNA from gel bands
  • Autoclaved glassware and supplies
  • Cleaned lab