IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/22

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July 22nd, 2010

  • Measured optical density of E. coli growing in M9 media in flasks twice
  • Placed order with Julie for Chelex
  • Miniprepped GFP and RFP for cotransformation
  • Quantified DNA from miniprep
  • Cotransformed RFP and GFP
  • Plated 20 ul of cotransformed RFP and GFP onto LB Carb Kanamycin plates
  • Started overnight cultures of GFP and TOP10
  • Took photos for iGEM WIKI