IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/15

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July 15th, 2010

  • Prepared E. coli with copper solutions in wider flasks for better oxygenation
  • Retested copper promoter constructs made from overnight cultures the night before and incubated at 37C for 4 hours
  • Aliquoted 1ml of the copper sulfate treated cultures to microcentrifuge tubes, centrifuged, and observed pellets for fluorescence with UV lamp
  • Compared optical density readings of the Copper sulfate treated cultures using various available spectrophotometers (Perkin Elmer Lambda 20, Thermo H, plate reader)
  • Rechecked RBS efficiencies of fluorescent proteins using RBS calculator
  • Discussed project description alterations
  • Discussed different wiki’s and own ideas for the site
  • Made more liquid cultures of E. Coli
  • Worked on web page design for the wiki
  • Investigated approaches to eliminate copper from media: Chelex, EDTA, M9 Minimal media