IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/14

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July 14th, 2010

  • Retested copper promoter construct (Added copper sulfate (5x10-4 M) to overnight cultures prepared from copper sulfate cultures that fluoresced the day before and incubated at 37C for 6 hours
  • Aliquoted 1ml of the copper sulfate treated cultures to microcentrifuge tubes, centrifuged, and observed pellets for fluorescence with UV lamp
  • Read fluorescence using the spectroflourometer
  • Finished project description and submitted it onto the wiki
  • Prepared E. coli with copper solutions- overnight cultures- in TB broth to retest copper promoter construct