IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/06

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July 6, 2010

  • Autoclaved glassware
  • Made Terrific Broth without antibiotics
  • Grew out CFP, YFP, and GFP
  • Did a transformation of RFP that has a tetracycline resistance (RFP biobrick into TOP10 cells)
  • Made solutions of copper solutions and TOP10 cells to determine optimal growth environment
  • Analyzed alignment utilizing BLAST of construct templates and primer sequences
  • Transformed YFP biobrick into TOP10 cells
  • Grew out YFP, CFP, and YFP from glycerol stock for testing of fluorescence using the plate reader in the morning
  • Contacted team about next regional meeting
  • Discussed skype calls and established set individuals on each team that would be in charge of maintaining communication over skype