IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/30

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June 30, 2010

  • Conducted a transformation of CFP (without the promoter region)
  • Prepared presentation for the VCU regional team meeting
  • Tested RFP in the plate reader and the spectroflourometer
  • Performed a co-transformation of TOP10 cells with two plasmids (PUC19 and PSB2K3)
  • Placed cells in copper solutions to test effect of copper on cell growth of E. coli containing the TOPO vector with PCR construct and incubated cultures overnight
  • Made CuSO₄ solutions