IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/24

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June 24, 2010

  • Took out 1 ml cell cultures of TOP10, CFP, RFP, and YFP
  • Read the OD of fluorescent samples
  • Utilized the plate reader
  • Read the plate reader data
  • Plated TOP10, YFP, and RFP
  • Prepared terrific broth of CFP
  • Took more photos for the wiki
  • Discussed plans for co-transformation
  • Analyzed fluorescent protein information on how to optimize the CFP
  • Used RBS calculator to discover an alternative RBS sequence to the CFP
  • Researched optimization
  • Investigated different potential backbones for putting the project together
  • Reviewing previous papers on USER fusion