IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/22

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June 22, 2010

  • Prepared TOP10 culture for fluorescence readings
  • Read optical density of fluorescence cultures and diluted to a common optical density- RFP and YFP diluted by using 100 ul of cells and 600ul of broth. CFP diluted by using 100ul of cells and 500 ul of broth
  • Read fluorescence with plate reader and subtracted out native fluorescence
  • Received Taq polymerase and new miniprep kit
  • Revision of Experimental design
  • Programmed thermocycler
  • Streaked 20 and 100ul of CFP, RFP, and YFP