IGEM:Virginia United/2009/Notebook/2010 UVA Lab/2010/06/29

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June 29th Lab Entry

  • Preparation of Arsenic Stock Solution
 1. Measure 1 mg of arsenic salt (Sodium (meta)Arsenite) 
 2. Measure 100 ml of distilled water 
 3. Mix the measured amount of arsenic salt with the measured distilled water
 (The concentration of this solution is about 10 mg/L)

For testing purposes, the stock solution is further dilluted to 1 mg/L.

  • Testing Arsenic Promoter
* Prepare five samples of 1ml cell culture 
* 5 ul, 10 ul, 50 ul, and 100 ul of Arsenic Stock Solution(1 mg/L) is added to four of the samples
 (The concentration of Arsenic in the samples are 5, 10, 50, and 100 ug/L.)

--Yong Y. Wu 14:37, 8 July 2010 (EDT)