IGEM:Virginia United/2009/Notebook/2010 UVA Lab/2010/06/08

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June 8th Lab Entry

LB Plates of Amplicillin were made in the morning:

 1. Measure 10 g of Bacto Tryptone, 10 g of NaCl, and 5 g of Bacto Yeast Extract
 2. Mixed with magnetic stirrer for 5 min
 3. 17 g of Bacto Agar Added
 4. Mixed with stirrer for another 5 min
 5. Autoclave for 45 min liquid cycle 
 6. The liquid is cooled for 40 min on a stir plate
 7. 1 ml Amplicillin stock solution (50(mg Amp)/ml H2O)) is added 

--Yong Y. Wu 11:30, 6 July 2010 (EDT)