IGEM:Virginia/2009/Notebook/VGEM2011/2011/08/12

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August 12th, 2011

  • Divided a URA DO plate into four quadrants and streaked each quadrant with one transformed yeast colony to provide further purification of the yeast collected. Plates were parafilmed and incubated at 25C.
  • Troubleshooting 13-22 pca reaction...

Ran all of the single oligos 13-62 on a gel to make sure the oligos exist:

1. 13

2. 14

3. 15

4. 16

5. 17

6. 58

7. 59

8. 60

9. 61

10. 62

  • Oligos 16, 17, 58, and 59 appeared as very faint bands. This means that we have a lot less DNA in the samples than we expected, which could have resulted in our failed pca reactions. [no image]
  • Ran 4 pca reactions: 13-58, 13-16, 17+58, 59-62. Ran the samples on the gel with the oligos that appeared faint in the other gel:

1. ladder

2. 16

3. 17

4. 58

5. 59

6. 13-58

7. 13-16

8. 17+58

9. 59-62

  • Still can’t see the oligos, but we have clear bands for 13-58 and 59-62. So we decided to run a second set of pca reactions. [no image]