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August 10th, 2011

  • Checked plates for mites.
  • PCR purified products from the day before and visualized the results on a gel:

1. Ladder

2. oligo mix for VEGF sequence

3. VEGF construct

4. amplified VEGF construct

  • Ran two more PCA reactions: one with oligos VEGF 1T-VEGF 12B and another with VEGF 13T-62. Then, we ran a second PCA combining the two segments. Finally, we amplifed the final construct.