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August 2nd, 2011

  • Prepared oligo mixes: 3 groups of 12 oligos (19-30; 31-42; 43-54) and 1 group of 8 oligos (55-62). Received the phusion polymerase and ran 4 PCA reactions. We found that the group of 8 oligos assembled well, but the groups of 12 did not assemble. We ruminated that the failure of assembly may be because there were too many oligos in the mixture.