IGEM:Virginia/2009/Notebook/VGEM2011/2011/07/28

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July 28th, 2011

  • Miniprepped the E. Coli and got concentrations of 1) 75.4, 2) 67.8, and 3) 76.9 ng/uL for each of the tubes of plasmid. Transformed yeast cells with the plasmids isolated through the mini-prep, plated, and incubated at 25C.
  • Re-ran reactions following the protocol outlined in the paper “PCR-based accurate synthesis of long DNA sequences.” Visualized DNA on gel:

1) ladder

2) PCA oligos 1-10

3) PCA oligos 11-20

4) PCA oligos 21-30

5) PCA oligos 31-40

6) PCA oligos 41-50

7) PCA oligos 51-62