IGEM:Virginia/2009/Notebook/VGEM2011/2011/07/26

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July 26th, 2011

  • Mini-prepped the DNA and nanodropped it:

1) 47.3 ng/uL

2) 39.3 ng/uL

3) 54.9 ng/uL

  • Ran the PCA with a 1:70 ratio of inner oligos to “primer” oligos. Ran the products on a gel and isolated the size we wanted.

These results are low but it was expected because results isolated on a gel tend to be low.

1) ladder

2) oligos 1-20 PCA

3) oligos 21-40 PCA

4) oligos 41-62 PCA