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June 30th, 2011

  • Tried the Gibson assembly with 5 oligos [6-10 and 11-15]. Purified the products and performed a nanodrop [6-10: 5.6ng/ul; 11-15: 5.7ng/ul] Ran the products on the gel and failed to get any results. Nanodropped the DNA stock solutions to verify there is an appropriate amount of DNA in the solutions.

1) Gibson assembly oligos 6-10 2) Gibson assembly oligos 11-15 3) Ladder