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June 29th, 2011

  • Cast another gel for gel electrophoresis. Jackie messed up the taking the first and second time so it had to be redone. Digested the Gibson and plasmid again. This time, the digestion was set for 2 hrs instead of 1 hr because the fastest recommended digestion time was 2 hrs. Also, the enzymes HindIII and SacI do not perform optimally in NEB buffer 2 so increasing the digestion time will allow for a more efficient digestion.

1) Ladder 2) Gibson assembly 3) Digested Gibson assembly 4) Digested plasmid