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June 28th, 2011

  • Determined the concentration of the assembled constructs from the Gibson assembly to be 31.0ng/uL using Nanodrop machine. Performed digestion on our Gibson constructs and plasmids. Cast gel for gel electrophoresis and ran the gel. Got plasmid backbone but no Gibson assembly DNA in the gel (see photo). Possible reasons for this discrepancy is Yanzhi’s spill with the master mix, Josh’s error in oligo mix, gel was left in the chamber for 2 hrs because of a power outage, and KpnI works better SacI but KpnI is not heat inactivated.