IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/07/27
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July 27, 2010
Took a look at the gel from yesterday of EGFP and MicF and was disappointed to see no bands in MicF and a 3 kB band in EGFP. Took a break from the treacherous EGFP and mini-preped a colony from each of the 4 transformations from 7/22/10. This was done using the Promega prep kit instructions. Using the nanodrop I got the following concentrations for my mini-preps:
Francis then ran a PCR to see if the insert was in the cells. I don't know where the procedure for that is though!