IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/06/22

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June 22, 2010

We went back into lab and tried to clone out MicA and MicF again. We conducted 40 uL reactions that included:

  • 10X Buffer 4 (uL)
  • 10mM dNTPs 1 (uL)
  • Template 2.5 (uL)
  • Forward Primer .4 (uL)
  • Reverse Primer .4 (uL)
  • Polymerase .4 (uL)
  • dH2O 31.3 (uL)
  • Total 40 (uL)

The template used was the MG1655 E.coli genome.