IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Lock and Key Decoder/2010/07/21

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2:30 p.m. - Performed 5 innoculations:

  • 5 mL LB Broth (x5 tubes)
  • 5 μL Kan (x2 tubes)
  • 10 μL Amp (x3 tubes)

Placed parts in tubes according to their resistances.


3:00 p.m. - Re-plated GFP onto LB+Amp because it was difficult to pick a single colony. (Forgot to re-sterilize inoculation loop before pulling it across previous streaks.)