IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Lock and Key Decoder/2010/07/19

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7/19

Transformation

  • Transformed 1 μL GFP plasmids from Steve's mini-prep into 100 μL competent cells.

Materials

  • 100 μL competent dH5α cells
  • 1 μL GFP plasmids
  • 1000 μL LB Broth

Plate

  • Plated Lock 1 (BBa_J01060) from glycerol stock onto Amp plate.
  • Plated Lock 3 (BBa-I714070) from glycerol stock onto Kan plate.
  • Plated RFP (BBa_J04650) from glycerol stock onto Amp plate.
  • Plated RFP (BBa_J04650) from glycerol stock onto Kan plate.
  • Left in 37°C room overnight.