IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Lock and Key Decoder/2010/07/14

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7/14/10

Transformation

  • Transformed 2μL of plasmids from well 12K, Plate 1 of the Spring 2010 DNA Distribution into a 100μL solution of competent cells.
  • Used new electroporation cuvette, which seemed to be differently shaped than old ones. (May have to use more solution for an efficient transformation)
  • Added 1000 μL LB broth, mixed, and placed cuvette labelled "GFP" into 37°C room for 1 hour.
  • Also placed 1 Amp and 1 Kan plate into 37°C room for 1 hour.

Plating

  • Spread 100μL of GFP transformed cells onto Amp plate and Kan plate from previous transformation.
  • Placed plates into 37°C room overnight.