IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/10/21
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Minipreps of golS + RBS, golB + RBS, and golT + RBS was conducted and each was eluted into 40 uL of H2O.
50 uL digestions of the following:
A gel was extracted of golS and golB.
The cell digestions were then ran on a gel and ligated. The ligations included:
Heat shock transformation was used to incorporate the plasmids into the E.coli.
An earlier miniprep was verified using PCR, which included:
Inoculations were made from the earlier cell transformation to be incubated overnight.