IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/10/04
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Today a digestion and ligation was done for golT to try and get it into the plasmid backbone pSBIC3 along with the terminator B0015. The ligation was transformed using heat shock and then incubated overnight in the 37 degree room.