IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/09/30

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9.30.10

GolS + Terminator, golB + Terminator, gesA + B0034 + pSBIC3, gesB + B0015 + pSBIC3, and gesC + B0015 + pSBIC3 were all ligated. After the ligations the cells were transformed using heat shock in 50 uL of cells.

The PCRs from yesterday were put into a gel and ran.

Another PCR was conducted to try and get golT out of the genome in a 50 uL reaction.