IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/09/29
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Verifications for golB + LamB, golS + LamB, EO420, E0430, E0240, pgolS + E0240, pgolS + E0420, pgolS + E0430, and golS + Terminator was done by PCR.
Digestions were also ran of golS + Terminator + pSBIT3, golb + Terminator + pSBIT3, RBS, gesA, gesB, gesC, and pSBIC3.
GolS + Terminator + pSBIT3 and golB + Terminator + pSBIT3 were sequenced using the VR primer.